Primers and PCR conditions used in this study.
- Ruoxi Chen (110319)
- Inderjit K. Barphagha (110320)
- Hari S. Karki (110322)
- Jong Hyun Ham (110324)
Publication date
December 2012
DOIAbstract
*<p>Restriction sites (underlined) introduced in primers: <u>GGTACC</u> (<i>Kpn</i>I), <u>GGATCC</u> (<i>Bam</i>HI), <u>ACTAGT</u> (<i>Spe</i>I), and <u>TCTAGA</u> (<i>Xba</i>I). Default PCR conditions were: initial denaturation, 95°C/5 min; denaturation, 94°C/30 s; number of cycles, 30; and final extension, 72°C/7 min.</p
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