Proteomic analysis.

<p>Representative image from three independent experiments of Coomassie-stained 2-DE patterns of DAOY(<b>A</b>), UW228 (<b>B</b>) and ONS-76 (<b>C</b>) cell lines. Proteins were separated using 7-cm, pH-3-10 NL strips followed by SDSPAGE on 10%, 7×10-cm gels. Proteins showing differential expression were indicated with different colors: in <i>blue</i> –proteins identified in all cell lines and listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063748#pone-0063748-t002" target="_blank">Table 2</a>; in <i>black</i>-proteins identified in adherent cells and spheres of each cell lines and in <i>red</i>-proteins only identified in spheres. Corresponding identifications are reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063748#pone-0063748-t003" target="_blank">Table 3</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063748#pone-0063748-t004" target="_blank">4</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063748#pone-0063748-t005" target="_blank">5</a>.