2-DE gel electrophoresis and Classification of differentially regulated proteins from proteomic analysis.

<p>Proteins were isolated from total protein of MSCs loaded onto the 2-DE gel electrophoresis. First dimension was 1 cm with a pH of 3 to 10 with NL IPG, and the second dimension was 10% gels (A: BM-MSCs, B: CB-MSCs, C: PB-MSCs). They were visualized using silver staining. The number indicates the position of up-regulated protein spots in the 2-DE pattern. (D) Ontological classification of differentially regulated proteins in terms of molecular functions using the Gene Ontology (<a href="http://www.geneontology.org" target="_blank">http://www.geneontology.org</a>) and UniProt (<a href="http://www.expasy.uniprot.org" target="_blank">http://www.expasy.uniprot.org</a>) websites. The composition of the identified proteins is presented as numbers for all individual identified proteins.