Stabilized γ-catenin suppresses neuronal differentiation in embryoid body assays and reduces overall tri-lineage differentiation efficiency.

<p>(A and B) Embryoid bodies were generated using control, β-catS33A, and γ-catS28A mESCs, and assayed for neuronal differentiation after 10 days. (A) Only WT EBs displayed immunofluorescent staining for the neuronal marker, β-III-tubulin. (B) The transcript levels of the neuronal markers <i>β-III-tubulin</i>, <i>Nestin</i>, <i>Map2</i>, <i>Tyrosine hydroxylase</i>, the endoderm marker <i>α-Fetoprotein</i>, and the mesoderm marker <i>Cardiac Troponin</i>, were reduced in EBs derived from mESCs overexpressing β-catS33A or γ-catS28A, as assessed by qRT-PCR analyses. Bars represent means and error bars indicate s.e.m. (n = 2). Size bar = 200 µm.