β-catenin autonomously regulates transcription in ventral neuronal progenitors.

<p>Coronal sections through midbrain of wt (a,c,e) and β-catenin^lof (b,d,f,g) embryos isolated at E10.5 (a,b), E11.5 (c,d) and E12.5 (e,f,g). Sections were co-stained with β-catenin (green), Lmx1a and TH (red) antibody. Asterisks mark β-catenin negative areas. Filled arrowheads indicate β-catenin+, Lmx1a+ cells in β-catenin^lof embryos. Cleared arrowheads point to β-catenin negative Lmx1a or TH expressing cells. V- ventricle. Tamoxifen was injected intraperitoneally into pregnant dams 48 hours prior to embryo isolation. 10 µm