Cumulative mortality of mud crab challenged with bacteria pre-coated with Sp-SPH protein.

<p>Crabs were challenged with <i>A. hydrophila</i>, <i>V. parahemolyticus</i> or <i>V. alginolyiicus</i> pre-coated with Sp-SPH protein as described above. Ten animals were used for each group. The mortality was recorded hourly. The cumulative mortality of crabs, in which the bacteria were coated with Sp-SPH protein (square, SPH), was compared with that of animals treated with “PBS-coated” bacteria (diamond, PBS). A: The cumulative mortality caused by <i>A. hydrophila</i>; B: The cumulative mortality caused by <i>V. parahemolyticus</i>; C: The cumulative mortality caused by <i>V. alginolyiicus</i>. This experiment was repeated three times and the data represented means of triplicates. Bars indicated mean ± S.E. (<i>n</i> = 3).