Distribution of GFAP in surface dividing VZ precursor cells during M-phase.

<p>Mitotic cells at the ventricular surface of the macaque occipital neocortex at E65 <i>(</i><b><i>A–C</i></b><i>)</i> and E80 <i>(</i><b><i>D</i></b><i>)</i> immunostained for the mitotic cell marker 4A4 (green) and GFAP (red), and costained with DAPI nuclear stain (blue). <i>(</i><b><i>A</i></b><i>)</i> 4A4+/GFAP+ prophase cell in the E65 macaque occipital cortex. During prophase there was strong GFAP immunoreactivity (IR) at the apical and basal poles of the cell. GFAP-IR often extended from the soma into the initial segment of the pial fiber on the basal pole of the cell to give the appearance of a “GFAP cap” (white arrowheads), and also labeled the pial fiber (white arrows). <i>(</i><b><i>B</i></b><i>)</i> 4A4+/GFAP+ metaphase cell in the E65 macaque occipital cortex. During metaphase GFAP encircled the nucleus in a relatively uniform pattern. The “GFAP cap” (white arrowheads) often appeared on one side of the condensed chromatin during metaphase. <i>(</i><b><i>C</i></b><i>)</i> 4A4+/GFAP+ anaphase cell in the E65 macaque occipital cortex. GFAP-IR surrounded the dividing chromosomes and was also present between the sister chromatids during anaphase. <i>(</i><b><i>D</i></b><i>)</i> 4A4+/GFAP+ telophase cell from E80 macaque occipital cortex. GFAP-IR surrounded the dividing sister chromatids of telophase cells and strong GFAP-IR was present in the cleavage furrow. Scale bar = 5 µm.