All mitotic cells at the ventricular surface of the macaque neocortical ventricular zone are GFAP+ and Pax6+ during cortical neurogenesis.

<p>Images show coronal sections of fetal macaque occipital neocortex immunostained with antibodies against phosphorylated vimentin (4A4, green) or phosphohistone H3 (PH3, green) to label all mitotic cells, and GFAP (red), and costained with DAPI (blue). <i>(</i><b><i>A</i></b><i>)</i> E65 macaque occipital cortex stained with 4A4 and GFAP antibodies. Panels <b><i>A1</i></b>, <b><i>A2</i></b>, and <b><i>A3</i></b> are magnified images of the numbered mitoses (white arrowheads) in Panel <b><i>A</i></b> showing colocalization of 4A4 and GFAP. <i>(</i><b><i>B</i></b><i>)</i> E80 macaque occipital cortex stained with PH3 and GFAP antibodies. Panel <b><i>B1</i></b> shows colocalization of PH3 and GFAP. <i>(</i><b><i>C</i></b><i>)</i> A coronal section of E80 macaque occipital cortex costained with antibodies against 4A4 (green), Pax6 (blue) and GFAP (red). All mitotic cells at the surface of the ventricle in the prenatal macaque neocortex expressed Pax6 and GFAP. Panels <b><i>C1</i></b>, <b><i>C2</i></b>, and <b><i>C3</i></b> show colocalization of 4A4, Pax6 and GFAP in surface mitoses. Scale bar in <b><i>A</i></b> = 25 µm, in <b><i>A1</i></b> = 5 µm, in <b><i>B</i></b> = 10 µm, in <b><i>C</i></b> = 10 µm.