Wnd mediates the functions of Hiw on dendritic growth.

<p>(A) Loss of <i>wnd</i> blocks dendrite reduction in <i>hiw</i> mutants, and ectopic Wnd restrains dendritic growth. Shown are representative dendrites of ddaC neurons, labeled by <i>ppk-CD4::tdTomato</i>, of the following genotypes: (1) <i>wt</i>; (2) <i>hiw^ΔN</i> homozygotes (<i>hiw</i>); (3) <i>wnd¹/wnd³</i>(<i>wnd</i>); (4) <i>hiw^ΔN</i>; <i>wnd¹/wnd³</i> double mutants (<i>hiw</i>; <i>wnd</i>); (5) overexpressing Wnd by <i>ppkGal4</i> (OE Wnd); (6) overexpressing a kinase dead form (K188A) of Wnd by <i>ppkGal4</i> (OE Wnd^KD). Scale bar, 50 µm. (B) Bar charts showing the quantification of total dendrite length (left) and number of dendrite termini (right). Samples of <i>wt</i> and <i>hiw^ΔN</i> that are used for statistical analysis are the same as those in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001572#pbio-1001572-g001" target="_blank">Figure 1</a>.