Inhibition of GRP78 induction by VCD.

<p>GRP78 protein expression was analyzed by Western blot analysis of MDA-MB-231 cells. (A) Cells were exposed to hypoglycemic (0.8 mM glucose) culture conditions for up to 48 h. Shown are two independent repeats (WB1, WB2) with different exposure times. (B) Cells were exposed to hypoglycemic culture conditions in the absence or presence of increasing concentrations of VCD for 24 h. Ctrl: cells were kept in normal (25 mM glucose) culture conditions. (C) Cells were treated with up to 1000 nM VCD under hypoglycemia or in the presence of 25 mM glucose for 24 h. In all cases, blots were re-probed with an antibody against actin (as a loading control).