The hippocampus of adult <i>Nfix</i>^+/− mice remains misshapen.

<p>No gross differences in the volume of hippocampal structures were detected between adult wild-type and <i>Nfix</i>^+/− mice (<b>A</b>) using magnetic resonance imaging at a resolution of 30 µm (<i>p</i>>0.5). Haematoxylin staining of coronal brain sections (<b>B</b> and <b>C</b>) revealed that the CA1 pyramidal cell layer (arrowhead in <b>C</b>) and dentate gyrus (arrow in <b>C</b>) remained misshapen in adult <i>Nfix^+/−</i> mice. Moreover the suprapyramidal blade of the dentate gyrus appeared thinner in adult <i>Nfix</i>^+/− mice (<b>C’</b>) compared with wild-type mice (<b>B’</b>). <b>B’</b> and <b>C’</b> are magnified views of the boxed regions in <b>B</b> and <b>C</b> respectively. Scale bar (in <b>C’</b>): <b>B</b>, <b>C</b> 500 µm; <b>B’</b>, <b>C’</b> 70 µm. Abbreviations: CA = Cornu ammonis; Py = pyramidal layer; Or = oriens layer; Rad = radiatum layer; MoDG = molecular layer of the dentate gyrus; GrDG = granule layer of the dentate gyrus; PoDG = polymorph layer of the dentate gyrus.