Heat map and hierarchical clustering.

<p>Individual miRNA expression were calculated by R platform and heat map was computed and described using a function of heatmap.2 in gplots. It uses hierarchical clustering with Euclidean distance; Pearson Linear Correlation and Ward's method to generate the hierarchical tree <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066086#pone.0066086-Ward1" target="_blank">[56]</a>. ANOVA was applied to extract differentially expressed miRNAs and adjustment of the p-value by multiple comparisons was performed by calculating FDR. Those miRNAs with FDR<0.1 were presented. The red indicates high level of miRNA expression and the blue shows low.