<p>MΦ (A) or PEDSV.15 cells representing an immortalized EDC line (B) were infected with either VRPΔE<sup>rns</sup>, CSFV, or CSFV-E<sup>rns</sup>Δ346 (MOI 5 TCID<sub>50</sub>/cell) and incubated for 48 h before co-culture with enriched pDC. The responses are compared to direct stimulation by CSFV or VRPΔE<sup>rns</sup>. Mean and standard deviation calculated from triplicate cultures are shown. The results are representative of two independent experiments.</p
<p>A) Freshly isolated BDCA1<sup>+</sup> mDCs were infected as indicated (MOI 50) and after 16 h all...
<p>Shown are the values of cytokine/chemokines (pg/ml) produced from the same source of PBMCs and da...
<p>A. pDC and mDC were sorted from Flt-3L induced BM-derived DC. 10 µg/ml of <i>L. lactis</i> JCM580...
<p>A and B. VRPΔE<sup>rns</sup>- (A) or CSFV- (B) infected SK-6 cells were co-cultured with enriched...
<p>A and B. Blocking of direct CSFV (A) or VRPΔE<sup>rns</sup> (B) activation of pDC by addition of ...
<p>A and B. IFN-α induction in pDC after stimulation with SK-6 or SK-6(E<sup>rns</sup>) cells infect...
<p>Enriched pDC were either stimulated with CSFV-infected SK-6 cells, VRPΔE<sup>rns</sup>-infected S...
<p>A. SK-6 cells were infected with VRPΔE<sup>rns</sup> for 24 h, washed and then treated with a DMS...
<p>Enriched pDC were either infected with CSFV, VRPΔE<sup>rns</sup> or co-cultured with CSFV- or VRP...
<p>IRS661 inhibits pDC activation by direct CSFV or VRPΔE<sup>rns</sup> infection (A) and by VRP-inf...
<p>BMDC obtained after 9 days of culture with GM-CSF were infected with CFSE-labeled metacyclic prom...
<p>HCMV-infected (MOI 50) pDCs (pDC+HCMV) and pDCs incubated with UV-inactivated virus (pDC+HCMV-UV)...
<p>Freshly isolated PBMCs stained with CFSE, treated with either RSV or mock conditioned media, and ...
<p>* P < 0.05 compared to pDLC. (A) PBMCs were incubated with FITC–dextran and analyzed by flow cyto...
<p>HCMV-infected (MOI 50) pDCs (pDC+HCMV) and pDCs incubated with UV-inactivated supernatant of HCMV...
<p>A) Freshly isolated BDCA1<sup>+</sup> mDCs were infected as indicated (MOI 50) and after 16 h all...
<p>Shown are the values of cytokine/chemokines (pg/ml) produced from the same source of PBMCs and da...
<p>A. pDC and mDC were sorted from Flt-3L induced BM-derived DC. 10 µg/ml of <i>L. lactis</i> JCM580...
<p>A and B. VRPΔE<sup>rns</sup>- (A) or CSFV- (B) infected SK-6 cells were co-cultured with enriched...
<p>A and B. Blocking of direct CSFV (A) or VRPΔE<sup>rns</sup> (B) activation of pDC by addition of ...
<p>A and B. IFN-α induction in pDC after stimulation with SK-6 or SK-6(E<sup>rns</sup>) cells infect...
<p>Enriched pDC were either stimulated with CSFV-infected SK-6 cells, VRPΔE<sup>rns</sup>-infected S...
<p>A. SK-6 cells were infected with VRPΔE<sup>rns</sup> for 24 h, washed and then treated with a DMS...
<p>Enriched pDC were either infected with CSFV, VRPΔE<sup>rns</sup> or co-cultured with CSFV- or VRP...
<p>IRS661 inhibits pDC activation by direct CSFV or VRPΔE<sup>rns</sup> infection (A) and by VRP-inf...
<p>BMDC obtained after 9 days of culture with GM-CSF were infected with CFSE-labeled metacyclic prom...
<p>HCMV-infected (MOI 50) pDCs (pDC+HCMV) and pDCs incubated with UV-inactivated virus (pDC+HCMV-UV)...
<p>Freshly isolated PBMCs stained with CFSE, treated with either RSV or mock conditioned media, and ...
<p>* P < 0.05 compared to pDLC. (A) PBMCs were incubated with FITC–dextran and analyzed by flow cyto...
<p>HCMV-infected (MOI 50) pDCs (pDC+HCMV) and pDCs incubated with UV-inactivated supernatant of HCMV...
<p>A) Freshly isolated BDCA1<sup>+</sup> mDCs were infected as indicated (MOI 50) and after 16 h all...
<p>Shown are the values of cytokine/chemokines (pg/ml) produced from the same source of PBMCs and da...
<p>A. pDC and mDC were sorted from Flt-3L induced BM-derived DC. 10 µg/ml of <i>L. lactis</i> JCM580...