siRNA-mediated cleavage of CD40 mRNA in vivo. Confocal fluorescence and brightfield images of siRNA internalization in DC.

<p>A) Schematic representation of the CD40 mRNA illustrating siRNA cleavage sites and RACE strategy to detect cleavage product. Agarose gel of RACE–PCR amplification showing specific cleavage products in different lupus nephritis samples (S1 and S2), and in vitro DC culture (S3 and S4). B) Quantification of renal internalization of Chol-siRNA administered iv/ip in lupus mice. C) Representative kidney photomicrographs (x400) of 1- Basal auto fluorescence, 2- iv administration, 3- ip administration. Data are expressed as mean±SEM of four separate experiments.*p<0.05 vs basal.