Functional analyses of fgt-1 on fat storage, dauer formation and brood size in <i>C. elegans</i>.

<p>A. The expression of the <i>fgt-1::egfp</i> fusion protein was detected by Western blot analysis using an anti-GFP antibody in the wild-type worms (wt) injected with (+) or without (-) the <i>fgt-1</i> double-stranded RNA (dsRNA, RNAi). B. The intensities of the fusion protein in A were quantified by Image Lab Software and were normalized to β-actin levels. Error bars represent SEM (n = 4). C, D, and E. Fat accumulation in the <i>fgt-1</i> RNAi wt (C) and <i>daf-2</i>(<i>e1370</i>) mutant worms (D), as well as in <i>fgt-1</i>(<i>tm3165</i>) mutant animals (E, no RNAi), was measured by Sudan black B staining. Staining intensity was quantitated using ImageJ software (NIH). Error bars represent SEM (n ≥ 41). Statistical analysis was conducted using Student’s <i>t</i>-test (**<i>P</i> < 0.01, ***<i>P</i> < 0.001). F and G. Comparison of the effects of <i>fgt-1</i> knockdown on the dauer formation rate (F) and brood size (G). In F, wild-type and <i>daf-2</i>(<i>e1370</i>) worms were incubated at either 20 ^°C or 25 ^°C. Error bars represent SEM (n ≥ 9 and 25 for F and G, respectively). Statistical analysis was conducted using Student’s <i>t</i>-test.