Dasatinib induces differentiation of NB4 cells.

<p>NB4 cells were treated with different concentrations of dasatinib for the indicated times. (A) The percentages of CD11b expression cells were determined as described. Data presented are the mean ± SD of three independent experiments. *** P<.001, ** P<.01 versus vehicle treated cells. (B) Cell cycle proportion was analyzed as described. Data presented are the mean ± SD of three independent experiments. *** P<.001, ** P<.01, */# P<.05 versus vehicle treated cells. (C) HL60 cells were treated with dasatinib (10 µM) for 72 h and NB4 cells were treated with dasatinib (5 µM) for 48 h. Cells were collected and cytospined onto slides, then stained with Wright–Giemsa stain. Stained cells were observed under microscope (×40). The data are representative of three independent experiments.