Selection of the infective dose for the<i>Plasmodium berghei</i> ED₉₀-normalized in vivo assay.

<p>Growth kinetics of <i>P. berghei</i> following intravenous infection of (A) immunocompetent CD1 or (B) immunodeficient NSG mice is shown. The plots show the parasitemia in peripheral blood of female mice infected with 0.1×10⁶, 1×10⁶, 10×10⁶, and 50×10⁶ infected erythrocytes. The dashed line (P₀) indicates the target human-equivalent parasitemia. Data are the mean ± standard deviation of n = 4 mice/group. Error bars are shown only if they are bigger than symbols.