CDC25B-induced centrin 2 foci accumulate other PCM components.

<p>Asynchronous U2OS-HA-CDC25B cells were incubated in the absence of tetracycline for 48-72 hours and then fixed and co-stained for centrin 2 (red), DAPI (blue) and either Ninein (A), Pericentrin (B), γ-tubulin (C), Nedd1 (D), PCM-1 (E) or polyglutamylated (polyglut.) tubulin (F), as indicated. Images were taken using an Appotome microscope, except for C, lower panel, which is a maximum intensity projection of a z-stack taken using a laser scanning confocal microscope, to enhance the detection of γ-tubulin at distant foci. Bar = 10 µm.