(A) Dox-inducible U2OS Tet-On cell lines carrying the indicated Cdc14B-GFPs and mock controls were treated with 1 μM Z-L-VS in the presence or absence of 4 μg/ml Dox for 48 h

Representative centrioles (red) were visualized by an anti-centrin antibody and DNA (blue) was visualized by DAPI. Bar, 5 μM. (B) The percentage of cells with more than four centrioles was calculated from the experiments shown in A and as indicated. Centrioles were counted in mock, uninduced controls (−Dox) and Cdc14B-GFP–induced (+Dox) cells (Cdc14B-GFP–positive at centrioles). All the data are shown as the means ± SD of three independent experiments. At least 400 cells were counted in each experiment.<p><b>Copyright information:</b></p><p>Taken from "Cdc14B depletion leads to centriole amplification, and its overexpression prevents unscheduled centriole duplication"</p><p></p><p>The Journal of Cell Biology 2008;181(3):475-483.</p><p>Published online 5 May 2008</p><p>PMCID:PMC2364701.</p><p>