SNX9 is required for efficient recruitment of CHC and TACC3 to the mitotic spindle.

<p>(A) Representative microscopy images illustrating the localization of CHC during interphase (Int) and the indicated mitotic stages in untreated cells and cells depleted of SNX9, SNX18 or SNX33 by siRNA. Met, metaphase. Ana, anaphase. Cyto, cytokinesis. Scale bars represent 10 µm. (B–C) The graphs represents the fluorescence intensity ratio of CHC at the mitotic spindle over the whole cell (B) and the overall fluorescence intensity of CHC within the whole cell (C) during metaphase (mean ± S.E.M., n > 16 per sample). ns, not significant; ***, <i>p</i> < 0.001 (One-way ANOVA). (D) Representative microscopy images demonstrating the localization of SNX9 (upper panels) in untreated and CHC-depleted HeLa cells during interphase, and metaphase. DNA shown in lower panels. Scale bars represent 10 µm. (E–F) Untreated, SNX9 and CHC-depleted metaphase HeLa cells were stained for TACC3. Representative microscopy images of TACC3 localization (upper panels) in these cells are shown in D. DNA shown in lower panels. Graphs represents the amount of TACC3 in each cell, expressed as (F) ratio of fluorescence intensity at the mitotic spindle compared to the whole cell and (G) the overall fluorescence intensity of TACC3 within the whole cell (mean ± S.E.M., n = > 6).