Localization in cells of Sts5 during the cell cycle.

<p>(<b>A</b>) Imaging of fission yeast cells co-expressing Sts5-3GFP and mCh-atb2 (labelling the different microtubule structures seen through the cell cycle, and hence acting as cell cycle stage indicators). Interphase cells (I) have diffuse Sts5 localization (with a few cytoplasmic speckles) while cells in mitosis (either in anaphase (A) or during the time of the post anaphase array (PAA)) have several Sts5 cytoplasmic dots. Scalebar: 5 µm. (<b>B</b>) Population based analysis of cycling cells revealed that at metaphase the number of Sts5 speckles greatly increases and sharply drops during septum formation. Average and standard deviation of number of dots were automatically detected in multiple cells (see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002732#s4" target="_blank">Materials and Methods</a> for details).