Mutation of NEDD9Y189 stimulates faster adhesion dynamics.

<p>A. Time-lapse microscopy of the assembly and disassembly of GFP-positive focal adhesions. Shown are examples of focal adhesions in NEDD9−/− MEFs transfected with GFP.NEDD9, GFP.NEDD9Y189F and GFP.NEDD9 Y214F. Each box shows a 4.7 µm×4.7 µm cropped region. Arrows point to each adhesion at the time of peak fluorescence intensity. B. Focal adhesion assembly rate constants (<i>k</i>) for the indicated fusion proteins. C. As for B, except data showing the disassembly rate constants (<i>k</i>). N>25 individual adhesions were analysed per condition. *p<0.05, N.S. = not significant.