NEDD9Y189D phospho-mimetic inhibits migration and increase focal adhesion lengths.

<p>A. Schematic representation of NEDD9 protein sequence showing the Y189D mutation. B. NEDD9−/− MEFs transfected with GFP.NEDD9 or GFP.NEDD9Y189D, as indicated. Arrows point to examples of positive focal adhesions. Scale bar = 20 µm. C. Average speed of NEDD9−/− fibroblasts transfected with the indicated GFP expression plasmids. Graphs show the average (n>100 cells per expression construct) and SEM. ***p<0.001. D. Fraction of focal adhesions versus adhesion length. Data for GFP.NEDD9 show in black circles (n = 112) and for GFP.Y189D in white circles (n = 96). E. Comparison of the fraction of focal adhesions less than 4 µm long. ***p<0.001.