Detection of WNV in brains of mice infected with WNV-NY99.

<p>(A) The amount of WNV positive-sense RNA copies present in the brains of mice between days 3 and 6 post-infection with WNV-NY99. Mice were euthanized on day 3, 4, 5 and 6 post-infection and brains were collected and homogenized in DMEM for RNA isolation. (B) The amount of positive-sense RNA copies present in the brains of control mice and mice inoculated on days 3 and 5 post-infection. Brains were collected in RNA-later for RNA isolation using Trizol. Histopathology of the brains of 9-day old C57BL/6 mice infected with WNV-NY99. (C) Infected neurons in the brain of a mouse euthanized on day 3 p.i. (objective 20x). (D) Infected neurons in the cortex of a mouse euthanized on day 5 p.i. (objective 20x). Pictures are representative of the number of infected cells found in the brain for the majority of mice.