Detection of peptide-specific T-cell responses by interferon-γ ELISPOT in rMVA vaccinated mice.

<p>Cellular immune responses in mice immunized with rMVA-N (A) or rMVA-Gn/Gc (B) measured by ELISPOT upon individual peptide stimulation of splenocytes. Values represent mean ± standard deviation (SD) of spot forming units (SFU) using spleen cells from two mice immunized with a single intraperitoneal dose of each rMVA. Peptides are numbered sequentially according to their position on their respective ORFs (see <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002309#pntd-0002309-t002" target="_blank">Tables 2</a> and <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002309#pntd-0002309-t003" target="_blank">3</a>). Data were transformed by subtraction of background (medium only) values obtained with no peptide incubation. Phytohemagglutinin (PHA) was used as a non-specific positive control for cell cytokine expression. A peptide corresponding to the pb9 T-cell epitope was used as recombinant antigen-specific positive control.