Analysis NP-specific cellular responses induced by vaccination.

<p>Splenocytes from immunized groups of mice were incubated for 24 or 48 h in the presence of rNP or peptides encompassing MHC class I and class II restricted epitopes of NP. The number of IFN-γ (A), IL-2 (B), IL-4 (C) and IL-17 (D) producing cells was then determined by ELISPOT. Results are expressed as number of spots of cells producing cytokines per 10⁶ spleen cells after subtraction of background values of unstimulated cells. The differences are statistically significant p<0.001 (***) compared to the results of cells obtained from mice vaccinated with rNP alone.