IFN-γ producing NK cells in response to mycobacteria express higher levels of CD56.

<p>Human NK cells were cultured in the presence of IL-2 (100U/ml) and/or <i>M. bovis</i> BCG (MOI 1:1) for a total of 24h including Brefeldin A treatment. A) FACS dot-plot showing the gating strategy to distinguish IFN-γ producing NK cells following BCG stimulation. B) CD56 overlay histogram comparing the expression of CD56 between IFN-γ producing and non-producing NK cells. C) Bar graph showing significant increase of CD56 mean fluorescence intensity (MFI) from IFN-γ producing NK cells following BCG stimulation across different donors (n=4, mean +/- SD, paired t test, **p<0.01).