Roles for the structure-specific nucleases in regulating crossover frequency and distribution.

<p>(A) Chromosome domains. Left tip, left arm, center, right arm and right tip are indicated according to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003586#pgen.1003586-Rockman1" target="_blank">[26]</a>. SNPs that alter the restriction length and are located closest to the boundary of each chromosome domain were chosen for chromosomes V and X. (B) Schematic representation of the strategy for obtaining the F2 progeny for snip-SNP analysis. Circles indicate hermaphrodites. Squares indicate males. White, Bristol. Black, Hawaiian. (C) The representative band pattern of PCR products at position “a” on the X chromosome. The restriction enzyme (<i>Bsp</i>HI) treatment was performed before loading. (D) Crossover frequencies and distributions observed in the entire a–d interval, left arm (a–b), center (b–c), and right arm (c–d) on chromosomes V and X. Highlighted cells indicate statistical difference compared to wild type (blue; P<0.05, grey; P<0.01, and yellow; P<0.001 by the Fisher's Exact Test and corrected by Sidak adjustment for multiple comparisons). Bold type indicates statistical significance (P<0.05) before Sidak correction.