Crossover Alteration in X Chromosome Asynapsis Backgrounds

<div><p>Two genotypes <i>(him-8, meDf2)</i> were assayed for recombination by genetic crossing and SNP mapping. (A) The genetic distance between two visible markers on chromosome III was assayed by genetic crosses. Map distance increased from 17 centimorgans in N2, to 29 centimorgans in both <i>him-8</i> and <i>meDf2</i>.</p><p>(B) Single-nucleotide polymorphism mapping of chromosomes II, III, and V. Five SNP markers were used, resulting in four intervals across the chromosome in which recombination could be assayed (<i>x</i> axis). The relative physical length of each region is shown by the distance between gray bars in the graph background.</p><p>(C) Physical and genetic locations of single-nucleotide polymorphisms analyzed. The horizontal bars represent the physical length of the chromosomes (II, III, and V), with polymorphisms indicated above, proportional to their physical distance. Below each bar the polymorphisms are traced to a horizontal dashed line representing the interpolated genetic distance between them, also indicated numerically in centimorgans. Labels for each interval, numbered 1–12, correspond between B and C.</p>