Mtb Ndk inhibits ROS dependent macrophage apoptosis.

<p><b>A</b>. RAW macrophages were infected for 48 h with the indicated Mtb strains at MOI of 10∶1. To block ROS production, gp91^phox peptide inhibitor (gp91 INH) or its scrambled version (gp91 SCR) were added at 50 µM 1 h prior to infection by Mtb Ndk-AS. Cells were then stained with Alexa Fluor 488-Annexin V and analyzed by confocal microscopy. Images are a merge of bright field, red fluorescence (Mtb DsRed strains), and green fluorescence (Annexin V). Values in the top right corner indicate the percentage of apoptotic cells. <b>B</b>. Macrophages were infected as described in A., and then stained for cleaved caspase 3 as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003499#s4" target="_blank">materials and methods</a>. Cells with active caspase 3 were identified and quantified by FACS. Data are representative of two independent experiments.