<i>CTCF</i> overexpression in WT, UFM and FXS fibroblasts.

<p><i>CTCF</i> overexpression in WT, UFM and FXS cell lines after transfection with a commercial vector containing the open reading frame of variant 1 of <i>CTCF</i>. Quantitative RT-PCR showed a strong increase of <i>CTCF</i> mRNA after 48 and 120 hours from transfection (<b>A</b>), while levels of <i>FMR1</i> transcription remained substantially unchanged (<b>B</b>). The levels of <i>CTCF</i> transcription in the untreated cells were arbitrarily set at 1 as well as those of <i>FMR1</i> transcript in WT and UFM fibroblasts, while those of <i>FMR1</i>-mRNA in FXS cells were set at 0. Bars indicate standard deviation.