PLA-695 reduces endothelial cell migration after radiation.

<p>HUVEC and bEND3 cells were plated on 60 mm plates and allowed to grow to 80% confluency. The semi-confluent cell layer was scraped using a 200 µl sterile pipette tip to create a scratch/wound devoid of cells. The remaining cells were treated with vehicle control or 300 nM PLA-695 for 45 min prior to IR with 3 Gy. Migration was assessed at 24 h after treatment. The number of cells migrated in the scratch/wound were counted and normalized to surrounding cell density per HPF. Shown are representative photomicrographs and bar graphs representing the mean percentages of migrating cells relative to corresponding controls with SEM from three experiments.