No expression of eMHC in RFs and attenuation of ongoing muscle regeneration in aged <i>mdx</i> and <i>mdx52</i> mice.

<p>(A) Triple staining of <i>mdx</i> and <i>mdx52</i> mice for RF (green), eMHC (red), and nucleus (blue). Revertant dystrophin is not co-localized with newly regenerated eMHC-positive fibers in TA and GC muscles from <i>mdx</i> and <i>mdx52</i> mice at any age. The pictures are representative GC muscles from <i>mdx</i> and <i>mdx52</i> mice at each age. 20x objective lens, scale bar = 100<b> </b>μm. (B) The number of eMHC-positive fibers. Values are mean ± S.D. (<i>n</i> = 3–6 mice per group). A significant decrease in the number of eMHC-positive fibers is found only at 18<b> </b>months old in <i>mdx</i> mice (**<i>P</i><0.01 compared to 2<b> </b>months old, †<i>P</i><0.05 compared to 6 and 12<b> </b>months old). Symbol colors are accordant with the color of mice (red; <i>mdx</i>, blue; <i>mdx52</i>).