Distinct changes in the percentage of centrally nucleated fibers by mutations and age in <i>mdx</i> and <i>mdx52</i> mice.

<p>(A) Representative images of TA muscles from <i>mdx</i> and <i>mdx52</i> mice at ages 2, 6, 12 and 18<b> </b>months with hematoxylin and eosin staining. Wild-type C57BL/6 muscle at 2<b> </b>months of age is displayed as a control. M: months. Scale bar = 100<b> </b>μm. (B) The percentage of centrally nucleated fibers in TA and GC muscles from <i>mdx</i> and <i>mdx52</i> mice. Three hundred to one thousand myofibers were counted in left and right muscles and the percentage of CNFs was averaged between the two muscles per mouse. Values are mean ± S.D. (<i>n</i> = 3–6 mice per group). *<i>P</i><0.05, **<i>P</i><0.01 between <i>mdx</i> and <i>mdx52</i> mice; †<i>P</i><0.05, ††<i>P</i><0.01 compared to 2<b> </b>months old; ‡<i>P</i><0.05, ‡‡<i>P</i><0.01 compared to 6<b> </b>months old. Symbol colors are accordant with the color of mice (red; <i>mdx</i>, blue; <i>mdx52</i>).