Polarisome tracking of directional change in Cdc42a (Green: Ste20 (Cdc42a), Red: Spa2, Blue: Bni1).

<p><b>Left: </b><i>In vivo</i> data. <b>Right: </b><i>In silico</i> data. <b>Top row:</b> In both the cell (<b>A</b>) and the simulation (<b>B</b>), the Cdc42a profile shifts first, followed by the the polarisome (indicated by Spa2). <b>Middle and bottom rows:</b> Spatial dynamics of Bni1 (<b>C, D</b>) and Spa2 (<b>E, F</b>) during polarisome tracking of Cdc42a. Note that the time scale of polarisome switching is similar between <i>in vivo</i> and <i>in silico</i> experiments, especially in the minute overlap time when two polarisomes are present. <b>Also see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003139#pcbi.1003139.s004" target="_blank">videos S3</a> and <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003139#pcbi.1003139.s005" target="_blank">S4</a>.</b>