Punctate Polarization (Green: Cdc42a, Red: Spa2).

<p>(<b>A</b>) Yeast cells treated with -factor show the wider Cdc42a (marked by Ste20-GFP) and tighter Spa2 polarization. (<b>B</b>) Visualization of a stochastic realization of the polarisome model (white indicates regions with actin cables attached to the membrane). (<b>C</b>) Normalized fluorescence intensity membrane profiles of Ste20-GFP and Spa2-mCherry from a yeast cell undergoing polarisome formation (Green: Ste20 (Cdc42a). Dashed black: Ste20 fit. Red: Spa2). Ensemble mean experimental data is in Fig. S19. (<b>D</b>) Normalized membrane intensity profile from stochastic and deterministic realizations of polarisome model, the Cdc42a input, and the mean output of a stochastic ensemble (). Inset: Absolute membrane intensity profile from stochastic and deterministic realizations of polarisome model, and Spa2 ensemble mean. (Red: Spa2 Stochastic. Dashed blue: Spa2 deterministic. Black diamond: Spa2 ensemble mean. Green: Cdc42a (input)).