<p>Randomly selected PSMA-targeted phage clones (C, E, G, H, J, L) from the third round of screening bound specifically to LNCaP cells (“L”), but not PC3 cells (“P”). While staining was preferentially bound at cell surface, there was evidence of internalization (scale 20 µm).</p
<p>5FAM-562 (1 µM, <i>A–C</i>, green) and 5FAM-563 (1 µM , <i>D–F</i>, green) bound and internalized...
<p>(<b>A</b>) Schematic representation of the <i>in vitro</i> and <i>in vivo</i> panning strategies ...
<p>(<b>A</b>) The clones shown are the ones for which the signal on HBVP-cells was highest, and for ...
<p><i>(A)</i> PSMA (100 nM) inhibited the binding of Clone 1 (ARLSHRPSYLLVCA) and Clone 2 (GTAVASRVY...
<p>(A) Lane 1, phage clone carrying C4 peptide; lane 2, phage clone carrying C1 peptide; Lane 3, pha...
<p>(A) after surface biopanning round 3 (B) after surface biopanning round 4A and (C) after solution...
<p>Plates were sensitized with a mAb directed against phage protein III (1 µg/ml) and 100 µl of puri...
<p>In the following pulse-chase experiments, HeLa cells (1×10<sup>6</sup> cells) in serum-free mediu...
<p>Unless otherwise specified, HeLa cells (1×10<sup>6</sup> cells) in serum-free medium were treated...
<p>(A). Seven positive phage clones were transferred to a PVDF membrane and a wild-type M13 phage wa...
<p>Phage clones binding to CHO-K1/VPAC1 cells (<i>blue bars</i>) and wild-type CHO-K1 cells (<i>red ...
<p>A) Monoclonal scFv screening ELISA testing 96 clones in each library. Bacterial supernatants were...
<p>(А) Fluorescence microscopy of MDA-MB-231 cells incubated with phage clone 1.1 and control bacter...
<p>Surface-bound 3035 (red color) and 1C1 (green color) were concurrently internalized in PC-3 cells...
<p>A and a, mock-infected FPRC cells; B and b, PCV2-infected FPRC cells. All cells were examined und...
<p>5FAM-562 (1 µM, <i>A–C</i>, green) and 5FAM-563 (1 µM , <i>D–F</i>, green) bound and internalized...
<p>(<b>A</b>) Schematic representation of the <i>in vitro</i> and <i>in vivo</i> panning strategies ...
<p>(<b>A</b>) The clones shown are the ones for which the signal on HBVP-cells was highest, and for ...
<p><i>(A)</i> PSMA (100 nM) inhibited the binding of Clone 1 (ARLSHRPSYLLVCA) and Clone 2 (GTAVASRVY...
<p>(A) Lane 1, phage clone carrying C4 peptide; lane 2, phage clone carrying C1 peptide; Lane 3, pha...
<p>(A) after surface biopanning round 3 (B) after surface biopanning round 4A and (C) after solution...
<p>Plates were sensitized with a mAb directed against phage protein III (1 µg/ml) and 100 µl of puri...
<p>In the following pulse-chase experiments, HeLa cells (1×10<sup>6</sup> cells) in serum-free mediu...
<p>Unless otherwise specified, HeLa cells (1×10<sup>6</sup> cells) in serum-free medium were treated...
<p>(A). Seven positive phage clones were transferred to a PVDF membrane and a wild-type M13 phage wa...
<p>Phage clones binding to CHO-K1/VPAC1 cells (<i>blue bars</i>) and wild-type CHO-K1 cells (<i>red ...
<p>A) Monoclonal scFv screening ELISA testing 96 clones in each library. Bacterial supernatants were...
<p>(А) Fluorescence microscopy of MDA-MB-231 cells incubated with phage clone 1.1 and control bacter...
<p>Surface-bound 3035 (red color) and 1C1 (green color) were concurrently internalized in PC-3 cells...
<p>A and a, mock-infected FPRC cells; B and b, PCV2-infected FPRC cells. All cells were examined und...
<p>5FAM-562 (1 µM, <i>A–C</i>, green) and 5FAM-563 (1 µM , <i>D–F</i>, green) bound and internalized...
<p>(<b>A</b>) Schematic representation of the <i>in vitro</i> and <i>in vivo</i> panning strategies ...
<p>(<b>A</b>) The clones shown are the ones for which the signal on HBVP-cells was highest, and for ...
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