Measurements of copy numbers for 24 drug-target genes by mrcPCR in NCI-60 cells.

<p>(A) PCR amplification products from each of six multiplex PCR (A, B, C, O, H, and H3) separated by 5% NuSieve® agarose gel electrophoresis. (B) Representative peaks from analysis of products of six single-base-extension reactions (A, B, C, O, H, and H3) in a control whole-blood DNA sample. Five peak pairs are shown for four target genes and one reference gene in each reaction. Each pair of peaks is from either genomic DNA (blue) or the competitor (red). (C) Heatmap of copy numbers for 24 genes in NCI-60 cells. Each copy number was measured three times and the mean values were used. Co, colon; Pr, prostate; N, NSCLC; Br, breast; Ov, ovarian; Me, melanoma; Re, renal; Le, leukemia.