mrcPCR assay for determination of <i>FCGR3A</i> and <i>FCGR3B</i> copies.

<p>(A) Alignment of wild-type and competitor sequences for <i>IGF1</i> and <i>FCGR3</i> used in mrcPCR assay. The nucleotides corresponding to modified bases are indicated in upper-case letters. The forward and reverse PCR primer sites are marked in brackets. EP1, EP2, and EP3 represent the extension primer sites, with the direction of the extension indicated by arrows. (B) Electrophoregram of extended products in mrcPCR assay. Extended nucleotides are indicated after the extension primers. Four peaks originated from genomic <i>IGF1</i> (EP1-C and EP2-T) and competitor <i>IGF1</i> (EP1-A and EP2-A), and the other three peaks originated from genomic <i>FCGR3A</i> (EP3-C), genomic <i>FCGR3B</i> (EP3-T), and competitor <i>FCGR3</i> (EP3-A). (C) Formula for the signal ratios (SRs) and the relative ratios (RRs) from peak heights of electrophoregram.