Localization of Piccolino at different types of ribbon synapses in the mouse.

<p><b>A</b>: Outer plexiform layer (OPL) of +/+ retina triple labeled with Pclo 49 (labels Piccolino; <i>green</i>), Pclo 44a (labels Pclo and Piccolino; <i>red</i>), and an antibody against CtBP2/RIBEYE (<i>blue</i>). <b>B</b>: Inner plexiform layer (IPL) of +/+ retina triple labeled with Pclo 49 (labels Piccolino; <i>green</i>), Pclo 44a (labels Pclo and Piccolino; <i>red</i>), and an antibody against CtBP2/RIBEYE (<i>blue</i>). Arrowheads point to ribbon synapses, arrows demarcate Pclo 44a single stained conventional chemical synapses. <b>C</b>: Inner hair cells (ihc) triple labeled with Pclo 49 (labels Piccolino; <i>green</i>), Pclo 44a (labels Pclo and Piccolino; <i>red</i>), and an antibody against CtBP2/RIBEYE (<i>blue</i>). Nuclei (stained with DAPI, not shown) are circled with dotted lines. Arrowheads point to ribbon synapses, arrows demarcate conventional chemical synapses. Scale bar in A,B: 10 µm, C: 5 µm.