Orthopox and parapox virus specific PCR assay results.

<p>Representative agarose gel electrophoresis of products from PCR assays to detect orthopox or parapox viral DNA. Assays used 50 ng of template from one representative skin sample and appropriate controls. Tracks: M - 1 kbp ladder; 1, 5, 9 - no template control; 2, 6, 10 - sample 41; 3, 7, 11 - vaccinia virus; 4, 8, 12 - sealpox virus. PCR products from the OPV-HA assay (tracks 1–4, predicted size 1138 bp), the PPV-DNApol assay (tracks 5–8, predicted size 536 bp) and the PPV-up/do assay (tracks 9–12, predicted size 84 bp).