<p>Representative agarose gels of PCR products from reactions using 50 ng DNA. <b>A</b>) DNA polymerase assay (predicted product size 543 bp); <b>B</b>) DNA topoisomerase I assay (predicted product size 344 bp). M - 100 bp ladder; track numbers are for sample numbers; 1–25 skin samples; 26 vaccinia virus; 27 sealpox virus; 28 no template control.</p
<p>A–C. The indicated amounts of <i>S. aureus</i> genomic DNA were subject to broad-range real-time ...
A<p>Each 25 µl PCR reaction contained the following: 1.25 µl of each primer from 10 µM stocks, 12.5 ...
BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture. Contaminating...
<p>Representative agarose gel electrophoresis of products from PCR assays to detect orthopox or para...
<p><b>A.</b> PCR using genomic DNA as template for four different PCR (PCR1 = 645bp, PCR3 = 517 bp, ...
<p>Lane M, 100-base-pair (bp) DNA ladder; lane 1, reference strain of <i>Escherichia coli</i>; lane2...
DNA polymerases are used in a plethora of biotechnical applications, especially in the polymerase ch...
<p>The agarose gel shows the amplification of PCR products from a linearized plasmid-only assay seri...
<p>L: 500 bp ladder, 1–4: patients' samples; 5: positive control; 6: negative control.</p
<p>The simulated culture assay series demonstrates that the relationship between assay template comp...
<p>Four dilutions of six DNA polymerase were tested with primers for 16S rDNA in the presence of 100...
<p>A: The PCR product amplified from the genomic DNA using the gB primers (Lane 1) and negative cont...
<p>Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DN...
<p>(<b>A</b>) DNA amplification with Phi29 DNA polymerase prepared by our procedure. (<b>B</b>) DNA ...
<p>M: size marker (GeneRuler 50–1000 bp DNA; Fermentas); CoV_1: HCoV-NL63, CoV_2: HCoV-HKU1; RSV: re...
<p>A–C. The indicated amounts of <i>S. aureus</i> genomic DNA were subject to broad-range real-time ...
A<p>Each 25 µl PCR reaction contained the following: 1.25 µl of each primer from 10 µM stocks, 12.5 ...
BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture. Contaminating...
<p>Representative agarose gel electrophoresis of products from PCR assays to detect orthopox or para...
<p><b>A.</b> PCR using genomic DNA as template for four different PCR (PCR1 = 645bp, PCR3 = 517 bp, ...
<p>Lane M, 100-base-pair (bp) DNA ladder; lane 1, reference strain of <i>Escherichia coli</i>; lane2...
DNA polymerases are used in a plethora of biotechnical applications, especially in the polymerase ch...
<p>The agarose gel shows the amplification of PCR products from a linearized plasmid-only assay seri...
<p>L: 500 bp ladder, 1–4: patients' samples; 5: positive control; 6: negative control.</p
<p>The simulated culture assay series demonstrates that the relationship between assay template comp...
<p>Four dilutions of six DNA polymerase were tested with primers for 16S rDNA in the presence of 100...
<p>A: The PCR product amplified from the genomic DNA using the gB primers (Lane 1) and negative cont...
<p>Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DN...
<p>(<b>A</b>) DNA amplification with Phi29 DNA polymerase prepared by our procedure. (<b>B</b>) DNA ...
<p>M: size marker (GeneRuler 50–1000 bp DNA; Fermentas); CoV_1: HCoV-NL63, CoV_2: HCoV-HKU1; RSV: re...
<p>A–C. The indicated amounts of <i>S. aureus</i> genomic DNA were subject to broad-range real-time ...
A<p>Each 25 µl PCR reaction contained the following: 1.25 µl of each primer from 10 µM stocks, 12.5 ...
BACKGROUND:PCR in principle can detect a single target molecule in a reaction mixture. Contaminating...