Expression and colocalization of neurons expressing mRNAs for GAD65 and GAD67 in the LS, Cg and MPOA.

<p>The number (A) and colocalization ratio (B) is presented. Note that mRNAs for both GAD65 and GAD67 are colocalized in the vast majority of neurons of the three brain regions. Each bar represents the mean+SEM obtained from six mice. Colocalization ratio of GAD67/GAD65 was calculated by dividing the number of neurons expressing both GAD65 and GAD67 mRNAs (colocalization) by total number of neurons expressing GAD65 mRNA, and multiplying by 100. A: ^a<i>p</i> < 0.001 <i>versus</i> LSr_D and LSr_I, ^b<i>p</i> < 0.05 <i>versus</i> LSc_D and LSc_I, ^c<i>p</i> < 0.001 <i>versus</i> LSc_D, ^d<i>p</i> < 0.001 <i>versus</i> LSr_D and LSr_I, ^e<i>p</i> < 0.05 <i>versus</i> LSc_D and LSc_I, ^f<i>p</i> < 0.001 <i>versus</i> LSc_D, ^g<i>p</i> < 0.001 <i>versus</i> GAD65 in LSr_V, ^h<i>p</i> < 0.001 <i>versus</i> GAD65 in LSc_V; B: ^a<i>p</i> < 0.05 <i>versus</i> LSc_D and LSc_I, ^b<i>p</i> < 0.05 <i>versus</i> GAD67/GAD65.