Expression and colocalization of neurons expressing mRNAs for GAD65/GAD67 (GAD) and immunoreactivity for calcium binding proteins in the LS, Cg and MPOA.

<p>Each bar represents the mean+SEM obtained from four mice. A: number of calbindin-immunoreactive cells: ^a<i>p</i> < 0.001 <i>versus</i> LSr_D and LSr_I, ^b<i>p</i> < 0.05 <i>versus</i> LSc_D and LSc_I, ^c<i>p</i> < 0.001 <i>versus</i> LSc_D, ^d<i>p</i> < 0.05 <i>versus</i> LSr_I and LSr_V, ^e<i>p</i> < 0.001 <i>versus</i> LSr_I and LSc_D, ^f<i>p</i> < 0.01 <i>versus</i> LSr_V, LSc_D and LSc_I; B: ratio of colocalization of GAD and calbindin. ^a<i>p</i> < 0.001 <i>versus</i> LSc_I, ^b<i>p</i> < 0.001 <i>versus</i> LSc_I and LSc_V, ^c<i>p</i> < 0.001 <i>versus</i> GAD/Calbindin; C: number of calretinin-immunoreactive cells, ^a<i>p</i> < 0.001 <i>versus</i> LSr_D and LSr_I, ^b<i>p</i> < 0.05 <i>versus</i> LSc_D and LSc_I, ^c<i>p</i> < 0.001 <i>versus</i> LSc_D, ^d<i>p</i> < 0.001 <i>versus</i> LSr_I and LSc_D, ^e<i>p</i> < 0.001 <i>versus</i> LSr_V and LSc_D; D, ratio of colocalization of GAD and calretinin, ^a<i>p</i> < 0.05 <i>versus</i> LSr_D and LSr_V, ^b<i>p</i> < 0.001 <i>versus</i> LSc_I and LSc_V, ^c<i>p</i> < 0.001 <i>versus</i> LSc_V, ^d<i>p</i> < 0.001 <i>versus</i> LSc_V, ^e<i>p</i> < 0.001 <i>versus</i> GAD/Calretinin.