Detection of active caspase-3 in the mesencephalic dopaminergic neurons.

<p>(a–j) Coronal paraffin brain sections of ApoER2/VLDLr^−/− (a–c), ApoER2^−/− (e–g) and VLDLr^−/− mice (h–J), stained with anti-active caspase-3 antibody (red) and TH (green). In all mutant mice (b, f, i) no positive signals for active caspase-3 were detected in SNc (a, e, h). (d) Positive control for active caspase-3: cells of intestinal villi, nuclei counterstained with Dapi (blue). (c, g, j) Merge of images in a, b; e, f, and h, i respectively. SN: Substania nigra, VTA: Ventral tegmental area. Scale bars = 200 µm.