CP NMJs have a reduced mitochondrial load.

<p>A) Confocal images showing double staining for acetylcholine receptor by α-bungarotoxin (red) and mitochondria (green) using a mouse monoclonal antibody for complex VI subunit I of cytochrome c oxidase (Mitosciences, Oregon). Images represent the mean intensities of fluorescence signal across 10 z-stacked images by confocal microscopy. CP NMJs show a decreased intensity for synaptic mitochondria. B) Average mitochondrial fluorescence intensities of NMJs of CP (93.25±8.017 SEM, n = 25 NMJs) and control (156.70±12.035 SEM, n = 25 NMJs) samples. Bars represent the mean ± SEM of immunofluorescence intensity in arbitrary fluorescence units based on the signal from the mitochondrial stain at the NMJs of CP and control samples.