Mitochondrial markers co-localizes poorly with synaptic markers in central presynapses.

<p>A) Brain sections were stained with anti-synaptophysin (green), anti-ATP synthase β subunit (red) antibodies, and a nucleic acid Hoechst stain (blue). Images were acquired on an LSM 7 Zeiss confocal microscope. The number of red punctae overlapping with the green were quantified from 10 different sections using the Image J co-localization plugin and were estimated to be 31% ± 5% for cortex, 28% ± 3% for cerebellum and 35% ± 6% for hippocampus. Data are represented as mean ± SEM; n = 10. Representative images are from the cortex (layer 2/3), cerebellum (molecular layer) and hippocampus (stratum radiatum) as depicted; the scale bars are 15 μm. B) Brain sections were stained with anti-bassoon (green), anti-Fis1 (red) antibodies, and a nucleic acid Hoechst stain (blue). Images were acquired on an LSM 7 Zeiss confocal microscope. The number of red punctae overlapping with the green were quantified from 4 different sections using the Image J co-localization plugin and were estimated to be 29% ± 2.5% for cortex, 32% ± 6% for cerebellum and 32% ± 8% for hippocampus. Data are represented as mean ± SEM; n = 6. Representative images are from the cortex (layer 2/3), cerebellum (molecular layer) and hippocampus (stratum radiatum) as depicted. Examples of higher magnification are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125185#pone.0125185.s002" target="_blank">S2 Fig</a>.