<p>Panel A: typical bands obtained from single individuals belonging to groups A or B, and of pools of two individuals of each group (A+B) with each set of primers. The rightmost and leftmost lanes used a 100 bp DNA ladder. Numbers indicate the expected sizes of the amplicons. Panels B and C: PCRs using generic ITS2 primers FCM/B1D and the primer set 3 (Cp135F / CpB315R / CpA425R), respectively, on single individuals from the 24 psyllid species described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072454#tab2" target="_blank">Table 2</a>.</p
<p>Genetic relationships between pre-existing minority drug resistance populations and majority-leve...
<p>A box-and-whisker plot is shown for each primer set separately for Ascomycota, Basidiomycota, and...
<p>Purified DNA (100 pg) from the indicated species was utilized as template in PCR reactions. Speci...
<p>(A) Detection limit of PCR F3-B3. Lane Amp, DNA of <i>Amphimerus</i> sp. (10 ng); lanes 10<sup>−1...
<p>This heatmap pairs with <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.012...
<p>List of primers for the assemblage-specific PCRs and sizes of the amplicons.</p
PCR parameters and expected amplicon sizes are shown. M13 and Illumina tails in Li primers and ECO V...
<p>Agarose gel (1.5%) with specific qPCR products of expected sizes. <b>A</b>: oligo(dT)- primed amp...
<p>Separate mock pools of ~2000 larvae were created consisting of equal proportions of 6 parasite sp...
<p>Primer sequences used for 454 sequencing are found in <a href="http://www.plosone.org/article/inf...
<p>The depicted gel shows the PCR products obtained from using the inferred oligonucleotide primer p...
<p>Predicted fragment sizes (approximate, in bp) and PCR cycle profiles (temperature in °C: time in ...
<p>(A) PCR verification of expected 206 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecul...
<p>Primers used for amplifying coding region of the respective genes by quantitative PCR and bisulfi...
<p>For the nested PCR, the first row for each gene depicts the primers used in the first and second ...
<p>Genetic relationships between pre-existing minority drug resistance populations and majority-leve...
<p>A box-and-whisker plot is shown for each primer set separately for Ascomycota, Basidiomycota, and...
<p>Purified DNA (100 pg) from the indicated species was utilized as template in PCR reactions. Speci...
<p>(A) Detection limit of PCR F3-B3. Lane Amp, DNA of <i>Amphimerus</i> sp. (10 ng); lanes 10<sup>−1...
<p>This heatmap pairs with <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.012...
<p>List of primers for the assemblage-specific PCRs and sizes of the amplicons.</p
PCR parameters and expected amplicon sizes are shown. M13 and Illumina tails in Li primers and ECO V...
<p>Agarose gel (1.5%) with specific qPCR products of expected sizes. <b>A</b>: oligo(dT)- primed amp...
<p>Separate mock pools of ~2000 larvae were created consisting of equal proportions of 6 parasite sp...
<p>Primer sequences used for 454 sequencing are found in <a href="http://www.plosone.org/article/inf...
<p>The depicted gel shows the PCR products obtained from using the inferred oligonucleotide primer p...
<p>Predicted fragment sizes (approximate, in bp) and PCR cycle profiles (temperature in °C: time in ...
<p>(A) PCR verification of expected 206 bp target length amplicon. Lane M, 50 bp DNA ladder (Molecul...
<p>Primers used for amplifying coding region of the respective genes by quantitative PCR and bisulfi...
<p>For the nested PCR, the first row for each gene depicts the primers used in the first and second ...
<p>Genetic relationships between pre-existing minority drug resistance populations and majority-leve...
<p>A box-and-whisker plot is shown for each primer set separately for Ascomycota, Basidiomycota, and...
<p>Purified DNA (100 pg) from the indicated species was utilized as template in PCR reactions. Speci...