S100A8 and S100A9 induce the secretion of several cytokines in human PBMCs.

<p>Cytokine production was evaluated using proteome profiler arrays (A and B) or ELISA (C), as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072138#s2" target="_blank">Materials and Methods</a>. Cells were stimulated for 24 hours, and supernatants were harvested and used for experiments. (B) Densitometry analyses of antibody arrays in PBMCs. Data represent supernatants pooled from 10 different donors (A and B). Black bars, upregulated cytokines; white bars, unchanged cytokines. Data are the mean ± SEM of at least five experiments performed on cells from different donors (C). ***p≤0.005, Dunnett’s multiple comparison test.