Effects of miR-650 expression on the survival pathway in SPC-A1/DTX cells.

<p>(<b>A</b>) Flow cytometric analysis of apoptosis in anti-miR-NC or anti-miR-650-transfected SPC-A1/DTX cells. (<b>B</b>) Hoechst staining analysis of apoptosis in anti-miR-NC or anti-miR-650-transfected SPC-A1/DTX cells. (<b>C</b>) Western blot detection of the expression of pro-caspase-3 and cleaved caspase-3 proteins in anti-miR-650 or pcDNA/ING4-transfected SPC-A1/DTX cells (anti-miR-NC or pcDNA/control was used as control). (<b>D</b>) Detection of caspase-3 activity in anti-miR-650 or pcDNA/ING4-transfected SPC-A1/DTX cells (anti-miR-NC or pcDNA/control was used as control). (<b>E</b>) Western blot analysis of the expression of Bcl-2 and Bax proteins in anti-miR-650 or pcDNA/ING4-transfected SPC-A1/DTX cells (anti-miR-NC or pcDNA/control was used as control). Equal loading was confirmed by showing equal GAPDH levels. Results represent the average of three independent experiments (mean±SD). *<i>P</i><0.05 or **<i>P</i><0.01, in comparison with anti-miR-NC or pcDNA/control-transfected cells.